Improving the Bleeding Protocol for Horseshoe Crabs With the Development of a Partially- Underwater Bleeding Apparatus

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Allender, M. C., Schumacher, J., George, R., Milam, J., & Odoi, A. (n.d.). The Effects of Short- and Long-Term Hypoxia on Hemolymph Gas Values in the American Horseshoe Crab (Limulus polyphemus) Using a Point-of-Care Analyzer. Journal of Zoo and Wildlife Medicine, 193-200. Retrieved from ResearchGate database.

Armstrong, P., & Conrad, M. (n.d.). Blood Collection from the American Horseshoe Crab, Limulus Polyphemus. Retrieved from

Bacterial Endotoxins Test. (n.d.). Retrieved March 14, 2019, from Nelson Labs website:

Hurton, L., & Berkson, J. (n.d.). Potential causes of mortality for horseshoe crabs (Limulus polyphemus) during the biomedical bleeding process. Fishery Bulletin, 104(2), 293-298.

Krisfalusi-Gannon, J., Ali, W., Dellinger, K., Robertson, L., Brady, T. E., Goddard, M. K. M., . . . Dellinger, A. L. (n.d.). The Role of Horseshoe Crabs in the Biomedical Industry and Recent Trends Impacting Species Sustainability. frontiers in Marine Science.

Lenka Hurton, Jim Berkson & Stephen Smith (2005) Estimation of total hemolymph volume in the horseshoe crab Limulus polyphemus, Marine and Freshwater Behaviour and Physiology, 38:2, 139-147, DOI: 10.1080/10236240500064354

Levin, J., Poore, T. E., Zauber, N. P., & Oser, R. S. (n.d.). Detection of Endotoxin in the Blood of Patients with Sepsis Due to Gram-Negative Bacteria. The New England Journal of Medicine. Retrieved from

Liu, J. S., & Passaglia, C. L. (n.d.). Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research. Retrieved from Jove website:

Mattei, J.H., Beekey, M.A., Potter, H.R., Bond, C.S., Woronik, A.R., Roberts, J.A., & Smith, K.A. (2011). Estimation of Short-Term Tag-Induced Mortality in Horseshoe Crabs Limulus polyphemus. Transactions of the American Fisheries Society, 140(4), 954-958.

Walls, E. A., & Berkson, J. (n.d.). Effects of Blood Extraction on the Mortality of the Horseshoe Crab, Limulus polyphemus. Virginia Journal of Science.


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