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Improving the Bleeding Protocol for Horseshoe Crabs With the Development of a Partially- Underwater Bleeding Apparatus

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June 27, 2020

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Appendix

Table 1:

Tank Number	Crab Number	Wet Body Weight (g)	Sex	Calculated Hemolymph Volume (ml)	Extraction Volume (mL)	Group
1	1	2.948	M	0.7582256	0.265379	Treatment
1	2	3.474	M	0.8935128	0.3127295	Treatment
2	1	1.701	M	0.4374972	0.153124	Treatment
2	2	4.264	M	1.0967008	0.3838453	Treatment
3	1	5.329	M	1.3706188	0.4797166	Treatment
3	2	3.594	M	0.9243768	0.3235319	Treatment
4	1	0.24	M	0.061728	0.0216048	Treatment
4	2	1.128	M	0.2901216	0.1015426	Treatment
5	1	0.368	M	0.0946496	0.0331274	Treatment
5	2	0.257	M	0.0661004	0.0231351	Treatment
5	3	0.292	M	0.0751024	0.0262858	Treatment
6	1	1.226	M	0.3153272	0.1103645	Treatment
6	2	1.334	M	0.3431048	0.1200867	Treatment
6	3	0.181	M	0.0465532	0.0162936	Treatment
7	1	0.639	M	0.1643508	0.0575228	Control
7	2	2.351	M	0.6046772	0.211637	Control
7	3	0.333	M	0.0856476	0.0299767	Control
8	1	0.41	M	0.105452	0.0369082	Control
8	2	1.175	M	0.30221	0.1057735	Control
9	1	0.769	M	0.1977868	0.0692254	Control
9	2	0.736	M	0.1892992	0.0662547	Control
10	1	3.679	M	0.9462388	0.3311836	Control
10	2	0.779	M	0.2003588	0.0701256	Control
11	1	2.637	M	0.6782364	0.2373827	Control
11	2	0.997	M	0.2564284	0.0897499	Control
12	1	0.801	M	0.2060172	0.072106	Control
12	2	1.011	M	0.2600292	0.0910102	Control

Table 1: Wet measured body weight, calculations for hemolymph volume, and calculations for extraction volume for each crab in their respective tanks.

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