Improving the Bleeding Protocol for Horseshoe Crabs With the Development of a Partially- Underwater Bleeding Apparatus

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Appendix

Table 1:
Tank NumberCrab NumberWet Body Weight (g)SexCalculated Hemolymph Volume (ml)Extraction Volume (mL)Group
112.948M0.75822560.265379Treatment
123.474M0.89351280.3127295Treatment
211.701M0.43749720.153124Treatment
224.264M1.09670080.3838453Treatment
315.329M1.37061880.4797166Treatment
323.594M0.92437680.3235319Treatment
410.24M0.0617280.0216048Treatment
421.128M0.29012160.1015426Treatment
510.368M0.09464960.0331274Treatment
520.257M0.06610040.0231351Treatment
530.292M0.07510240.0262858Treatment
611.226M0.31532720.1103645Treatment
621.334M0.34310480.1200867Treatment
630.181M0.04655320.0162936Treatment
710.639M0.16435080.0575228Control
722.351M0.60467720.211637Control
730.333M0.08564760.0299767Control
810.41M0.1054520.0369082Control
821.175M0.302210.1057735Control
910.769M0.19778680.0692254Control
920.736M0.18929920.0662547Control
1013.679M0.94623880.3311836Control
1020.779M0.20035880.0701256Control
1112.637M0.67823640.2373827Control
1120.997M0.25642840.0897499Control
1210.801M0.20601720.072106Control
1221.011M0.26002920.0910102Control
Table 1: Wet measured body weight, calculations for hemolymph volume, and calculations for extraction volume for each crab in their respective tanks.

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